The Investigative Services Division of the Nebraska State Patrol includes three areas of concentration, drug investigation, criminal investigation and liquor enforcement The Investigative Services Division in Troop B- Norfolk has one Lieutenant, three Sergeants and 11 Investigators. ISO personnel are stationed in Norfolk, Columbus, Albion, Plainview and South Sioux City. Civilian employees are also a critical component of the Investigative Services Division helping with intelligence gathering, evidence and report processing.

Criminal Investigators in Troop B work cases ranging from theft to homicides. Troop B Investigators are actively involved in investigative crimes involving assault, child abuse and Internet Crimes Against Children (ICAC).

The Troop B Drug Division is an integral component of the S.N.A.R.E. Drug Task Force comprised of city, county and federal investigators, analysts and prosecutors as well as the Sioux City DEA Task Force based in Sioux City, Iowa. Premise inspections and compliance checks are also conducted on liquor license holders within the Troop B area of coverage.

Troop B investigators also monitor licensed liquor establishments to verify that they are in compliance with the State's Liquor laws. Enforcement of the laws pertaining to gaming, tobacco and underage drinking are also a part of this division's duties.